Ethanol extracts of Scutellaria baicalensis protect against lipopolysaccharideinduced acute liver injury in mice
To investigated the protective potential of ethanol extracts of
Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide
(LPS)-induced liver injury. Methods: Dried roots of S. baicalensis were
extracted with ethanol and concentrated to yield a dry residue. Mice
were administered 200 mg/kg of the ethanol extracts orally once daily
for one week. Animals were subsequently administered a single dose of
LPS (5 mg/kg of body weight, intraperitoneal injection). Both protein
and mRNA levels of cytokines, such as tumor necrosis factor alpha,
interleukin-1β, and interleukin-6 in liver tissues were evaluated by
ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric
oxide synthase, and nuclear factor-kB protein levels in liver tissues
were analyzed by western blotting. Results: Liver injury induced by LPS
significantly increased necrosis factor alpha, interleukin-1β,
interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and
nuclear factor-κB in liver tissues. Treatment with ethanol extracts of
S. baicalensis prevented all of these observed changes associated with
LPS-induced injury in liver mice. Conclusions: Our study showed that S.
baicalensis is potentially protective against LPSinduced liver injury in
mice.
Title:
| Ethanol extracts of Scutellaria baicalensis protect against lipopolysaccharideinduced acute liver injury in mice | |
| Authors: | Nguyen, Thanh Hai Pham, Thi Minh Hue Le, Tuan Anh Vu, Duc Loi Duong, Thi Ly Huong Nguyen, Huu Tung Dang, Kim Thu Bui, Thanh Tung |
| Keywords: | alcohol antiinflammatory activity Scutellaria baicalensis Lipopolysaccharide Liver injury Nuclear factor-κB |
| Issue Date: | 2015 |
| Publisher: | Hainan Medical University |
| Citation: | Scopus |
| Abstract: | Objective: To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. Methods: Dried roots of S. baicalensis were extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS (5 mg/kg of body weight, intraperitoneal injection). Both protein and mRNA levels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-kB protein levels in liver tissues were analyzed by western blotting. Results: Liver injury induced by LPS significantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensis prevented all of these observed changes associated with LPS-induced injury in liver mice. Conclusions: Our study showed that S. baicalensis is potentially protective against LPSinduced liver injury in mice. |
| Description: | Asian Pacific Journal of Tropical Biomedicine, Volume 5, Issue 9, April 20, 2015, Pages 761-767 |
| URI: | http://www.sciencedirect.com/science/article/pii/S2221169115001410 http://repository.vnu.edu.vn/handle/VNU_123/32406 |
| ISSN: | 22211691 |
| Appears in Collections: | Bài báo của ĐHQGHN trong Scopus |
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